Francesca Di Leva
e-mail: dileva AT science.unitn.it
affiliation: Università di Trento
research area(s): Cancer Biology
Course:
Biomolecular Sciences
University/Istitution: Università di Trento
University/Istitution: Università di Trento
February 2011 to present : Postdoctoral fellow in the laboratory of Prof. Alessandro Quattrone at the Centre for Integrative Biology (CIBIO), University of Trento- Italy .
January 2009 to January 2011 : Postdoctoral fellow in the laboratory of Prof. Paolo Macchi at the Centre for Integrative Biology (CIBIO), University of Trento- Italy . Identification of post-translation modifications in Staufen, Barentsz and Pumilio RNA-binding proteins.
29th march to 5th april 2009: visiting scientist at the Center for Brain Research, Medical University of Vienna, Vienna
October 2005 to December 2008: Postdoctoral fellow in the laboratory of Dr.Marisa Brini at the Sperimental Veterinary Sciences Department, University of Padua- Italy . Study of PMCA activity in cellular and murine models
October 2003 to September 2005: Postdoctoral fellowship with Prof. Elio Marciano at the “Institute of Audiology, Department of Neurosciences and Behavioural Sciences” at the “Federico II” University of Naples- Italy. Genetic analysis of haematic and salivar samples derived from patients affected by hereditary hearing loss. Functional studies of mammalian promoter of sybl-1 gene
May 2000 to April 2003: PhD student with Prof. Elio Marciano at the “Institute of Audiology, Department of Neurosciences and Behavioural Sciences” at the “Federico II” University of Naples - Italy and in the laboratory of Dr. Michele D’Urso at the Institute of Genetics and Biophysics, IGB A. Buzzato Traversi – Naples - Italy.
Thesis title:” Otosclerosis: a genetic analysis in italian affected families”.
Linkage analysis of families affected by otosclerosis. Characterization of non subtractive cDNA library from guinea pig inner ear
September 1999 to September 2000: Trainee in the laboratory of Dr. Michele D’Urso. Genetic analysis of DNA sample from patients affected by x-linked mental retardation (MRX)
March 1998 to July 1999. Undergraduate Student in the laboratory of Prof. Giusseppe Scala at the Biochemistry and Biotechnology Department of the University Federico II of Naples.
Thesis title ”Physical and Functional interaction of HIV-1 Tat with E2F-4, a transcriptiojnal regulator of mammalian cell cycle”. Final grades:110 cum laude /110. Functional studies of TAT protein from HIV virus and characterization of its interaction with E2F4 protein in model cells.
Teaching experience
Undergraduate and PhD students advisor
2009-10 to 2009-11 and 2010-10 to 2010-11: Practical exercitation in Molecular Biology at the University of Trento
EDUCATION
October 2003 to October 2006. Master in “Nutritional Sciences”. Final grade 50 cum laude/50 at the Federico II University in Naples, Italy
May 2000 to April 2003. PhD in “Physiopathology of Audio Verbal Communication” at the Federico II University in Naples, Italy
Second session of year 2000. Biologist qualification
October 1993 to July 1999. Biology degree. At the Federico II University in Naples. Final grade 110 cum laude/110
January 2009 to January 2011 : Postdoctoral fellow in the laboratory of Prof. Paolo Macchi at the Centre for Integrative Biology (CIBIO), University of Trento- Italy . Identification of post-translation modifications in Staufen, Barentsz and Pumilio RNA-binding proteins.
29th march to 5th april 2009: visiting scientist at the Center for Brain Research, Medical University of Vienna, Vienna
October 2005 to December 2008: Postdoctoral fellow in the laboratory of Dr.Marisa Brini at the Sperimental Veterinary Sciences Department, University of Padua- Italy . Study of PMCA activity in cellular and murine models
October 2003 to September 2005: Postdoctoral fellowship with Prof. Elio Marciano at the “Institute of Audiology, Department of Neurosciences and Behavioural Sciences” at the “Federico II” University of Naples- Italy. Genetic analysis of haematic and salivar samples derived from patients affected by hereditary hearing loss. Functional studies of mammalian promoter of sybl-1 gene
May 2000 to April 2003: PhD student with Prof. Elio Marciano at the “Institute of Audiology, Department of Neurosciences and Behavioural Sciences” at the “Federico II” University of Naples - Italy and in the laboratory of Dr. Michele D’Urso at the Institute of Genetics and Biophysics, IGB A. Buzzato Traversi – Naples - Italy.
Thesis title:” Otosclerosis: a genetic analysis in italian affected families”.
Linkage analysis of families affected by otosclerosis. Characterization of non subtractive cDNA library from guinea pig inner ear
September 1999 to September 2000: Trainee in the laboratory of Dr. Michele D’Urso. Genetic analysis of DNA sample from patients affected by x-linked mental retardation (MRX)
March 1998 to July 1999. Undergraduate Student in the laboratory of Prof. Giusseppe Scala at the Biochemistry and Biotechnology Department of the University Federico II of Naples.
Thesis title ”Physical and Functional interaction of HIV-1 Tat with E2F-4, a transcriptiojnal regulator of mammalian cell cycle”. Final grades:110 cum laude /110. Functional studies of TAT protein from HIV virus and characterization of its interaction with E2F4 protein in model cells.
Teaching experience
Undergraduate and PhD students advisor
2009-10 to 2009-11 and 2010-10 to 2010-11: Practical exercitation in Molecular Biology at the University of Trento
EDUCATION
October 2003 to October 2006. Master in “Nutritional Sciences”. Final grade 50 cum laude/50 at the Federico II University in Naples, Italy
May 2000 to April 2003. PhD in “Physiopathology of Audio Verbal Communication” at the Federico II University in Naples, Italy
Second session of year 2000. Biologist qualification
October 1993 to July 1999. Biology degree. At the Federico II University in Naples. Final grade 110 cum laude/110
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Mario Bortolozzi, Marisa Brini, Nick Parkinson, Giulia Crispino, Pietro Scimemi, Romolo, Daniele De Siati, Francesca Di Leva, Andrew Parker, Saida Ortolano, Edoardo Arslan, Steve, Brown, Ernesto Carafoli2, Fabio Mammano “the novel pmca2 pump mutation tommy impairs cytosolic calcium clearance in hair cells and links to deafness in mice” J. of Biol. Chem. 2010, 258(48):37693-703.
Brini M, Di Leva F, Ortega CK, Domi T, Ottoini D, Leonardi E, Tosatto SC, Carafoloi E “Deletions and mutations in the acidic lipid-binding region of the plasma membrane Ca2+ pump: a study on different splicing variants of isoform2” J. of Biol. Chem. 2010, 285(40):30779-91.
Chinetti Viviana; Iossa, Sandra; Auletta, Gennaro; Laria, Carla; De Luca, Maria; Di Leva, Francesca; Riccardi, Pasquale; Giannini, Pasquale; Gasparini, Paolo; Ciccodicola, Alfredo; Marciano, Elio; Franzé, Annamaria “Screening for GJB2 and GJB6 gene mutations in patients from Campania region with sensorineural hearing loss” International Journal of Audiology, Int. J. Audiol, 2010, 49(4):326-331.
John Vessey, Ewald Gingl, Lucia Schoderboeck, Julia Riefler, Ettore Luzi, Francesca Di Leva, Daniela Karra and Paolo Macchi “Mammalian Pum2 regulates dendrite morphogenesis and synaptic function” PNAS 2010, 107(7):3222-7.
Sarah L Spiden#, Mario Bortolozzi#, Francesca Di Leva#, Martin Hrabé de Angelis, Helmut Fuchs, Dmitry Lim, Saida Ortolano, Neil J. Ingham, Marisa Brini, Ernesto Carafoli, Fabio Mammano and Karen P Steel “The Novel Mouse Mutation Oblivion Inactivates the PMCA2 Pump and Causes Progressive Hearing Loss” PLoS Genet. 2008, 4(10). joint first authors
Francesca Di Leva, Teuta Domi, Laura Fedrizzi, Dmitry Lim, Ernesto Carafoli. The plasma membrane Ca2+ ATPase of animal cells: Structure, Function and Regulation. Arch Biochem Biophys. 2008, 476(1):65-74.
Ernesto Carafoli, Laura Fedrizzi, Teuta Domi, Francesca Di Leva and Marisa Brini. CALCIUM PUMPS . Handbook of cell signaling 2nd edition, in press.
Linde C., Di Leva F., Domi T., Tosatto S.C.E., Brini M., Carafoli C “inhibitory interaction of the 14-3-3 protein with ubiquitous (PMCA1) and tissue specific (PMCA3) isoforms of Plasma membrane Ca2+ pump” Cell Calcium 2007, 43(6): 550-561.
Brini Marisa, Di Leva Francesca, Domi Teuta, Fedrizzi Laura, Lim Dmitry and Carafoli Ernesto “Plasma-membrane calcium pumps and hereditary deafness” Biochem Soc Trans. 2007, 35(Pt 5):913-8.
Teuta Domi, Francesca Di Leva, Laura Fedrizzi, Alessandro Rimessi , Marisa Brini “Functional specificity of PMCA isoforms?” Ann N Y Acad Sci. 2007, 1099:237-246.
Ficarella, R.†, Di Leva, F.†, Bortolozzi, M.†, Ortolano, S.†, Donaudy, F., Petrillo, S. Melchionda, M., Lelli, A., Domi, T., Fedrizzi, L., Lim, D., Shull, G.E., Gasparini, P., Brini, M., Mammano, F., Carafoli, E. “A functional study of plasma membrane calcium-pump isoform 2 mutants causino digenic deafness” †These authors have contributed equally. PNAS 2007, 104 (5):1516-1521.
Di Leva F, D'Adamo P, Cubellis M V, D'Eustacchio A, Errichiello M, Saulino C, Auletta G, Giannini P, Donaudy F, Ciccodicola A, Gasparini P, Franzè A and Marciano E “Identification of a Novel Mutation in the Myosin VIIA Motor Domain in a Family with Autosomal Dominant Hearing Loss (DFNA11) Audiol Neurootol. 2006, 11(3):157-164.
Annamaria Franzé, Antonella Caravelli, Francesca Di Leva, Elio Marciano, Gennaro Auletta, Federica D’Aulos, Claudio Saulino, Laura Esposito, Massimo Carella and Paolo Gasparini ”Audiometric evaluation for carriers of the connexin 26 mutation 35delG” Eur Arch Otorhinolaryngol. 2005, 262(11):921-4.
Brini M, Di Leva F, Ortega CK, Domi T, Ottoini D, Leonardi E, Tosatto SC, Carafoloi E “Deletions and mutations in the acidic lipid-binding region of the plasma membrane Ca2+ pump: a study on different splicing variants of isoform2” J. of Biol. Chem. 2010, 285(40):30779-91.
Chinetti Viviana; Iossa, Sandra; Auletta, Gennaro; Laria, Carla; De Luca, Maria; Di Leva, Francesca; Riccardi, Pasquale; Giannini, Pasquale; Gasparini, Paolo; Ciccodicola, Alfredo; Marciano, Elio; Franzé, Annamaria “Screening for GJB2 and GJB6 gene mutations in patients from Campania region with sensorineural hearing loss” International Journal of Audiology, Int. J. Audiol, 2010, 49(4):326-331.
John Vessey, Ewald Gingl, Lucia Schoderboeck, Julia Riefler, Ettore Luzi, Francesca Di Leva, Daniela Karra and Paolo Macchi “Mammalian Pum2 regulates dendrite morphogenesis and synaptic function” PNAS 2010, 107(7):3222-7.
Sarah L Spiden#, Mario Bortolozzi#, Francesca Di Leva#, Martin Hrabé de Angelis, Helmut Fuchs, Dmitry Lim, Saida Ortolano, Neil J. Ingham, Marisa Brini, Ernesto Carafoli, Fabio Mammano and Karen P Steel “The Novel Mouse Mutation Oblivion Inactivates the PMCA2 Pump and Causes Progressive Hearing Loss” PLoS Genet. 2008, 4(10). joint first authors
Francesca Di Leva, Teuta Domi, Laura Fedrizzi, Dmitry Lim, Ernesto Carafoli. The plasma membrane Ca2+ ATPase of animal cells: Structure, Function and Regulation. Arch Biochem Biophys. 2008, 476(1):65-74.
Ernesto Carafoli, Laura Fedrizzi, Teuta Domi, Francesca Di Leva and Marisa Brini. CALCIUM PUMPS . Handbook of cell signaling 2nd edition, in press.
Linde C., Di Leva F., Domi T., Tosatto S.C.E., Brini M., Carafoli C “inhibitory interaction of the 14-3-3 protein with ubiquitous (PMCA1) and tissue specific (PMCA3) isoforms of Plasma membrane Ca2+ pump” Cell Calcium 2007, 43(6): 550-561.
Brini Marisa, Di Leva Francesca, Domi Teuta, Fedrizzi Laura, Lim Dmitry and Carafoli Ernesto “Plasma-membrane calcium pumps and hereditary deafness” Biochem Soc Trans. 2007, 35(Pt 5):913-8.
Teuta Domi, Francesca Di Leva, Laura Fedrizzi, Alessandro Rimessi , Marisa Brini “Functional specificity of PMCA isoforms?” Ann N Y Acad Sci. 2007, 1099:237-246.
Ficarella, R.†, Di Leva, F.†, Bortolozzi, M.†, Ortolano, S.†, Donaudy, F., Petrillo, S. Melchionda, M., Lelli, A., Domi, T., Fedrizzi, L., Lim, D., Shull, G.E., Gasparini, P., Brini, M., Mammano, F., Carafoli, E. “A functional study of plasma membrane calcium-pump isoform 2 mutants causino digenic deafness” †These authors have contributed equally. PNAS 2007, 104 (5):1516-1521.
Di Leva F, D'Adamo P, Cubellis M V, D'Eustacchio A, Errichiello M, Saulino C, Auletta G, Giannini P, Donaudy F, Ciccodicola A, Gasparini P, Franzè A and Marciano E “Identification of a Novel Mutation in the Myosin VIIA Motor Domain in a Family with Autosomal Dominant Hearing Loss (DFNA11) Audiol Neurootol. 2006, 11(3):157-164.
Annamaria Franzé, Antonella Caravelli, Francesca Di Leva, Elio Marciano, Gennaro Auletta, Federica D’Aulos, Claudio Saulino, Laura Esposito, Massimo Carella and Paolo Gasparini ”Audiometric evaluation for carriers of the connexin 26 mutation 35delG” Eur Arch Otorhinolaryngol. 2005, 262(11):921-4.
No projects are available to students for the current accademic year.